The games are presented primarily from a side-scrolling perspective. Players guide members of Mystery Inc. through themed stages, search for clues and useful objects, overcome hazards, and avoid or confront enemies while advancing each mystery.

The compilation contains two separate campaigns, one based on Scooby-Doo and the other on Scooby-Doo 2: Monsters Unleashed. Progression comes from completing stage objectives, collecting investigation-related items, and reaching new areas. Exact mechanics and level layouts vary between the two included games.

Search each area carefully before moving on. Clues and objects can be easy to overlook, and exploring side paths often helps reveal the route forward.

Avoid unnecessary contact with enemies and hazards. Learn the timing of moving obstacles, preserve health where possible, and use the environment rather than rushing through unfamiliar sections.

When a stage presents an investigation objective, check nearby rooms and platforms systematically instead of assuming the most visible path contains every required item.